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Protein characterisation

Bioanalysis & Characterisation

You can rely on us to make the product that you need – but to finish the job, it is also often important to prove that the correct product has been made, and to characterise its key properties.

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Therefore – as you would expect – Fleet Bioprocessing Ltd. possess extensive knowhow in the selection and execution of suitable analytical procedures. Of course, we are experts in immunoassay development and we frequently develop immunoassays for product characterisation purposes as well as for diagnostic applications. But we also have an extensive arsenal of physicochemical techniques available to provide comprehensive characterisation information for conjugates and other products.

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Here are some of the techniques at our disposal, either in-house or through our trusted partners:

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  • Size Exclusion Chromatography (SEC)

  • Affinity Chromatography

  • Hydrophobic Interaction Chromatography (HIC)

  • Ion Exchange Chromatography (IEC)

  • Thin Layer Chromatography (TLC)

  • Mass Spectrometry (MS)

  • Liquid Chromatography-Mass Spectrometry (LC-MS)

  • UV-Visible Spectrophotometry

  • Fluorescence Spectrometry

  • Luminescence Spectrometry

  • Gel Electrophoresis (including SDS-PAGE, 2D gels, Difference Gel Electrophoresis (DIGE) etc.)

  • Western Blotting

  • Nuclear Magnetic Resonance (NMR) Spectroscopy

  • Circular Dichroism (CD) Spectroscopy

  • Particle Sizing and Charge Determination

  • Melting Point Determination

  • Elemental Analysis

  • ICP Atomic Emission Spectroscopy (ICP-AES)

  • Differential Scanning Calorimetry (DSC)

  • Thermal Gravimetric Analysis (TGA)

  • Moisture Content Determination

  • Microbiological Testing (including bioburden analysis)

  • Enzyme Activity Measurement

  • Biotin Incorporation Determination

  • ELISA/Immunoassay Testing

  • Affinity Determination (Kd) for Antibodies and Other Ligand Binders

  • Epitope Mapping

  • Antibody Isotype Determination

  • Surface Plasmon Resonance (SPR) eg Biacore

  • Bio-Layer Interferometry (BLI) eg Octet

  • Impurity Determination eg Host Cell Protein (HCP) Analysis

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